Deleted
Sample Discontinued
Experience: None
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Coding

Part:BBa_K2114016:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-09)


CgeA_G4S_HA_aGFPnano


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.


Source

The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala et al. [Ref]

References